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Down-regulation of TRPC3 channels abolished the BDNF-induced elevation of intracellular Ca2+ in rodent microglial cells. The average traces showing the effect of BDNF (20 ng/ml) on intracellular Ca2+ mobilization in 6-3 microglial cells transfected with TRPC3-targeting siRNA (black line) and scrambled control siRNA (gray line). The average trace was determined from 10 representative traces of intracellular Ca2+ in each condition.
