Skip to main content
. 2014 May 14;289(26):18239–18257. doi: 10.1074/jbc.M113.546044

TABLE 1.

PCR and RT-PCR primers used in this study

Gene Forward primer sequence (5′ to 3′) Reverse primer sequence (5′ to 3′)
Sf-1 GTATCCTCGAGCTATGGACTATTCGTACGACGAGG GACATGAATTCTCAAGTCTGCTTGGCCTGCAGC
E2f1 GTATGAATTCTATGGCCGTAGCCCCCGCGG CTAGGTCGACTCAGAAATCCAGAGGGGTCAGG
Sf-1 3′UTR GCTTACTCGAGTGCCTCCAAAAGACTCCTTG GTATAGCGGCCGCTTATAAGGGCACTTCTTGTC
E2f1 3′UTR CTACTCTCGAGCAGAAGCCTAGGGATTCAGGGTG CGAGCGCGGCCGCCATCAAAACATTTATTAAAATCAAAG
MiR-320 promoter(−2941/−48) GTATCGAGCTCTCCACCTCCATATCTTCTTCCT GTATCCTCGAGACCGTCGGATAAATACTATGGTC
MiR-320 promoter(−2019/−48) GTATCGAGCTCTGTGAGCCAGTAAGTACACTAC GTATCCTCGAGACCGTCGGATAAATACTATGGTC
miR-320 promoter(−1282/−48) GTATCGAGCTCATCTCTAGGCCACACAACCTAA GTATCCTCGAGACCGTCGGATAAATACTATGGTC
MiR-320 promoter(−998/−48) GTATCGAGCTCACATCTACTGTTACACACATAAC GTATCCTCGAGACCGTCGGATAAATACTATGGTC
MiR-320 promoter(−2019/−48) mutant form GTGCTCATGACTGCAACAGTCCTGGGA ACTGTTGCAGTCATGAGCACATCGAAA