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A minimal domain containing the last 96 amino acids of Loz1 is sufficient to confer zinc-dependent repression of adh4. A, schematic diagram of pZ-loz1GFP truncations. See the legend to Fig. 5A for details. B and C, loz1Δ cells expressing the constructs shown in A were grown in ZL-EMM with or without a 200 μm zinc supplement. Cells were harvested, and total RNA was extracted and subjected to RNA blot analysis (B), or crude protein extracts prepared for immunoblot analysis (C).
