FIGURE 7.

pZ-MtfAGFP is regulated by zinc in loz1Δ cells. A, an alignment of the double zinc finger domains from Loz1 and MtfA. B, schematic diagram of Loz1/MtfA chimeras. Loz1 sequences are shown in light gray, and MtfA sequences are shown in dark gray. Numbers represent amino acid number in the respective proteins. C and D, loz1Δ cells expressing the constructs shown in B were grown in ZL-EMM with or without a 200 μm zinc supplement. Cells were harvested, and total RNA was extracted for RNA blot analysis (C), or crude protein extracts were prepared for immunoblot analysis (D).