TABLE 1.
Allele | DNA mutation | Amino acid substitution | Isolation condition |
---|---|---|---|
loz1-1 | C1528G | R510G | Spontaneous mutation identified via transformationa |
loz1-4 | T1430C | F477S | 10 μg/ml antimycin A |
loz1-5 | G865T | G289Stop | 10 μg/ml antimycin A |
loz1-8 | G1418A | C473Y | 10 μg/ml antimycin A |
loz1-15 | T1404G | Y468Stop | 10 μg/ml antimycin A |
loz1-K5 | T1408C | C470R | Spontaneous mutation in YES medium |
loz1-23 | T1417G | C473G | 5 μg/ml antimycin A |
loz1-25 | C1466T | S489F | 5 μg/ml antimycin A |
loz1-K25 | G298T | E100Stop | 10 μg/ml antimycin A |
loz1-K26 | C1558G | H520D | 10 μg/ml antimycin A |
loz1-K27 | C931T | Q311Stop | 10 μg/ml antimycin A |
loz1-K29 | C952T | Q318Stop | 10 μg/ml antimycin A + 500 μm zinc |
loz1-K30 | C874T | Q292Stop | 10 μg/ml antimycin A + 500 μm zinc |
loz1-K31 | G1436T | R479M | 10 μg/ml antimycin A |
loz1-K33 | G1539A | M513I | 10 μg/ml antimycin A + 500 μm zinc |
loz1-K34 | C889T | Q297Stop | 10 μg/ml antimycin A + 500 μm zinc |
loz1-K35 | C1327T | R443Stop | 10 μg/ml antimycin A + 500 μm zinc |
loz1-K36 | T666G | Y222Stop | 10 μg/ml antimycin A + 500 μm zinc |
loz1-K40 | C1456T | H486Y | 10 μg/ml antimycin A |
a The original loz1-1 allele reported in Ref. 6.