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. 2014 May 15;289(26):18087–18096. doi: 10.1074/jbc.M114.551333

TABLE 1.

loz1 alleles containing a point mutation

Allele DNA mutation Amino acid substitution Isolation condition
loz1-1 C1528G R510G Spontaneous mutation identified via transformationa
loz1-4 T1430C F477S 10 μg/ml antimycin A
loz1-5 G865T G289Stop 10 μg/ml antimycin A
loz1-8 G1418A C473Y 10 μg/ml antimycin A
loz1-15 T1404G Y468Stop 10 μg/ml antimycin A
loz1-K5 T1408C C470R Spontaneous mutation in YES medium
loz1-23 T1417G C473G 5 μg/ml antimycin A
loz1-25 C1466T S489F 5 μg/ml antimycin A
loz1-K25 G298T E100Stop 10 μg/ml antimycin A
loz1-K26 C1558G H520D 10 μg/ml antimycin A
loz1-K27 C931T Q311Stop 10 μg/ml antimycin A
loz1-K29 C952T Q318Stop 10 μg/ml antimycin A + 500 μm zinc
loz1-K30 C874T Q292Stop 10 μg/ml antimycin A + 500 μm zinc
loz1-K31 G1436T R479M 10 μg/ml antimycin A
loz1-K33 G1539A M513I 10 μg/ml antimycin A + 500 μm zinc
loz1-K34 C889T Q297Stop 10 μg/ml antimycin A + 500 μm zinc
loz1-K35 C1327T R443Stop 10 μg/ml antimycin A + 500 μm zinc
loz1-K36 T666G Y222Stop 10 μg/ml antimycin A + 500 μm zinc
loz1-K40 C1456T H486Y 10 μg/ml antimycin A

a The original loz1-1 allele reported in Ref. 6.