FIGURE 6.

Endocytosis of Arr1-eGFP is characterized by the presence of cytosolic vesicles in dissociated photoreceptors. Dissociated photoreceptors isolated from pupae or adult that were pretreated either with or without blue light for 4 min were imaged. Blue light treated pupal photoreceptors contain numerous cytosolic vesicles enriched with Arr1-eGFP (A and B). Without blue light, Arr1-eGFP was not sequestered in vesicles but was localized in rhabdomeres and cytoplasm of pupal photoreceptors (C). In isolated adult photoreceptors, Arr1-eGFP was preferentially localized in rhabdomeres with (D and E) or without prior blue light treatment (F). Scale bar, 10 μm.