Table 1.
Primer* | Sequence |
---|---|
SJ TRECs forward | 5’-CAC ATC CCT TTC AAC CAT GCT-3’ |
SJ TRECs reverse | 5’-TGC AGG TGC CTA TGC ATC A-3’ |
SJ KRECs forward | 5’-TCC CTT AGT GGC ATT ATT TGT ATC ACT-3’ |
SJ KRECs reverse | 5’-AGG AGC CAG CTC TTA CCC TAG AGT-3’ |
TCRAC forward | 5’-TGG CCT AAC CCT GAT CCT CTT-3’ |
TCRAC reverse | 5’-GGA TTT AGA GTC TCT CAG CTG GTA CAC-3’ |
Probe* | Sequence |
TRECs probe | 5’-FAM-ACA CCT CTG GTT TTT GTA AAG GTG CCC ACT-TAMRA-3’ |
KRECs probe | 5’-HEX-TCT GCA CGG GCA GCA GGT TGG-TAMRA-3’ |
TCRAC probe | 5’-FAM-TCC CAC AGA TAT CCA GAA CCC TGA CCC-TAMRA-3’ |
*Sottini et al.54