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. 2013 May 1;2(1):9–16. doi: 10.4081/jphr.2013.e3

Table 1.

Primer and probe sequences for TREC and KREC quantification.

Primer* Sequence
SJ TRECs forward 5’-CAC ATC CCT TTC AAC CAT GCT-3’
SJ TRECs reverse 5’-TGC AGG TGC CTA TGC ATC A-3’
SJ KRECs forward 5’-TCC CTT AGT GGC ATT ATT TGT ATC ACT-3’
SJ KRECs reverse 5’-AGG AGC CAG CTC TTA CCC TAG AGT-3’
TCRAC forward 5’-TGG CCT AAC CCT GAT CCT CTT-3’
TCRAC reverse 5’-GGA TTT AGA GTC TCT CAG CTG GTA CAC-3’
Probe* Sequence
TRECs probe 5’-FAM-ACA CCT CTG GTT TTT GTA AAG GTG CCC ACT-TAMRA-3’
KRECs probe 5’-HEX-TCT GCA CGG GCA GCA GGT TGG-TAMRA-3’
TCRAC probe 5’-FAM-TCC CAC AGA TAT CCA GAA CCC TGA CCC-TAMRA-3’

*Sottini et al.54