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Overview of the SpeedyGenes protocol using oligonucleotide ‘spiking’ to generate protein libraries. Oligonucleotides encoding the variant (mixed base) sequences are used to synthesise the intermediate blocks in the first step of the gene synthesis protocol. However, the variant sequences are heavily digested during the endonuclease incubation, hence the oligonucleotides encoding the variant sequences are ‘spiked’ into the final PCR to synthesise the full-length gene variant.
