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. 2014 May 12;23(18):4945–4959. doi: 10.1093/hmg/ddu210

Figure 1.

Figure 1.

Expression of an expanded CGG-repeat reporter in AWC neurons. (A) Representation of the GFP reporter containing expanded CGG repeats. GFP reporters, carrying a human FMR1 5′UTR fragment with 0, 30, or 99 CGG repeats, were expressed under an AWC-specific promoter (pAWC); these reporters are referred to as 0CGG, 30CGG and 99CGG. The open reading frame for GFP was followed by a 3′UTR element from unc-54. Arrow denotes the start site of transcription. (B) Expression of the GFP reporter in the AWC neuron. (Top) Schematic diagram of the AWC neuron in the head of C. elegans. Inset represents the area visualized in confocal florescence images below. The anatomy of the AWC neuron is diagramed in an enlarged cartoon in the left dotted box. (Bottom) Confocal florescence images of transgenic animals expressing GFP in the AWC neuron. GFP is expressed either from the FMR1 5′UTR without CGG repeats (left) or with 99 CGG repeats (right). The AWC neuron was identified by expression of DsRed from an AWC-specific promoter (pstr-2) and the anatomy is labeled in the 99CGG animal. Anterior is left for both images.