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. 2014 Jul 29;136(33):11582–11585. doi: 10.1021/ja505305z

Figure 1.

Figure 1

Catalytic domain of Tet1 can catalyze the formation of 5-hmrC from 5-mrC in RNA in vitro. (a) Tet-catalyzed formation of 5-hmrC. (b) HPLC traces for the separation of the nucleoside mixtures of single-stranded RNA, UUUCAGCUC(5-mrC)GGUCACGCUC, without Tet1 treatment and the same RNA after the Tet1-mediated oxidation. The peaks corresponding to 5-hmrC, 5-mrC, and canonical nucleosides are labeled. (c,d) MS/MS and MS/MS/MS characterizations of 5-hmrC, which monitor the fragmentation of the [M + H]+ ion of the 5-hmrC (c) and the further fragmentation of the protonated nucleobase (d), respectively. Displayed in the inset of (c) is the positive ion electrospray ionization mass spectrum for 5-hmrC.