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. 2014 Jul 29;136(33):11582–11585. doi: 10.1021/ja505305z

Figure 2.

Figure 2

LC-MS for monitoring the Tet1-mediated oxidation of 5-mrC in a single-stranded RNA, AGCUC(5-mrC)GGUCA (left) and a duplex DNA, d(AGCTC(5-mdC)GGTCA) /d(TGACCGGAGCT) (right). Shown are the higher resolution “ultra-zoom-scan” MS results for monitoring the [M – 3H]3– ions of the initial 5-mC-bearing 11mer RNA (left) or DNA (right), together with their oxidation products, where the 5-mC is oxidized to 5-hmC, 5-foC, and 5-caC. The peaks at around m/z 1166 and 1117 for the control samples in the left and right panels are attributed to the Na+ ion adduct, i.e., the [M + Na+ – 4H]3– ions, of the 5-mrC-containing RNA and 5-mdC-bearing DNA strand, respectively.