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. 2014 Jul 10;25(8):1511–1516. doi: 10.1021/bc5002455

Figure 2.

Figure 2

Characterization of PrA-PEG-SA adaptor assembly on a solid support and in solution. (a) Conjugation of PrA to biotin-PEG-NH2 was performed on a solid support (monomeric avidin resin) and in solution. SDS-PAGE characterization of products reveals high purity of mono-PEG-PrA conjugates produced via solid-phase bioconjugation, in contrast to a heterogeneous mixture of PrA with 1, 2, 3, 4+ PEG molecules produced by bioconjugation in solution. Molecular weight reference is shown in kDa. (b) Conjugation of PrA-PEG-biotin to SA on a solid support (IgG agarose) and in solution. Characterization with hybrid gel demonstrates 1:1:1 PrA-PEG-SA stoichiometry achieved with solid-phase procedure, whereas mixed SA conjugates with 0, 1, 2, 3+ PrA-PEG-biotin are produced via assembly in solution.