Figure 3.

Characterization of adaptor binding functionality with ELISA. (a) Test schematic. IgG-coated surface was incubated with PrA(G,L)-PEG-SA, then labeled with biotinylated HRP via binding to available SA sites on IgG-bound PrA(G,L)-PEG-SA adaptors. (b) IgG concentration-dependent signal detected with PrA-PEG-SA adaptors confirms preserved functionality of both PrA and SA blocks, whereas only background signal detected in control (simple mixture of adaptor components) shows lack of nonspecific binding.