(A) RP-LC-ESI-MS base peak intensity (BPI) chro-matogram and 2D overlaid BPI plot of reduced and permethy-lated N-glycans derived from 100 ng of RNase B. The insets are the mass spectra of each peak. (B) Extracted ion chromatograms (EICs) of the same sample analyzed by RP-LC-MALDI-MS. These traces were generated using Microsoft excel and smooth fitting of ion count data. Symbols: blue square, N-acetylglucosamine; green circle, mannose; red triangle, fucose; yellow circle, galac-tose, magenta diamond, N-acetylneuraminic acid.