Diagram of the response obtained in the sensorgrams of the binding of ATIII (500 nM) with modified heparosan after 3 min injection. Enzymatic modifications: A (N-sulfoheparosan modified with C5-epi), AB (N-sulfoheparosan modified with C5-epi and 2-OST), ABC (N-sulfoheparosan modified with C5-epi, 2-OST, and 6-OST), ABCD (N-sulfoheparosan modified with C5-epi, 2-OST, 6-OST and 3-OST), ABD (N-sulfoheparosan modified with C5-epi, 2-OST, and 3-OST), AC (N-sulfoheparosan modified with C5-epi, and 6-OST). For a better comparison a relative response (RU of the binding/RU of the polysaccharide immobilization) has been used.