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. Author manuscript; available in PMC: 2014 Aug 21.
Published in final edited form as: Semin Thromb Hemost. 2007 Jul;33(5):453–465. doi: 10.1055/s-2007-982076

Figure 3.

Figure 3

Schematic synthesis of sulfonated polysaccharides and 3′-phosphoadenosine-5′-phosphosultate (PAPS) regeneration system. (A) The stepwise enzymatic synthesis of sulfonated polysaccharides using heparan sulfate (HS) sulfotransferases. The description of intermediate polysaccharides is shown in the text. Compounds 4a and 4b were prepared by inverting the order of sulfonation steps. 4a was prepared by incubating compound 1 with 2-O-sullotransferase (2-OST) followed by 6-OST, whereas 4b was prepared by incubating compound 1 with 6-OST followed by 2-O5T. (B) The reaction catalyzed by arylsulfotransferase IV to generate PAPS.