Table 2.
Summary of the Disaccharide Compositions of the Synthetic Polysaccharides*
| Compound | ΔUA-GlcNAc (nmole)† (%) |
ΔUA-GlcNS (nmole) (%) |
ΔUA2S-GlcNS (nmole) (%) |
ΔUA-GlcNS6S (nmole) (%) |
ΔUA2S-GlcNS6S (nmole) (%) |
Total Recovery (%)‡ |
|---|---|---|---|---|---|---|
| 1 | 12.5 (16.1%) | 50.0 (64.4%) | 6.3 (8.1%) | 6.3 (8.1%) | 2.5 (3.2%) | 54.0 |
| 2 | 12.5 (16.6%) | 27.5 (36.6%) | 30.0 (40.0%) | 2.5 (3.3%) | 2.5 (3.3%) | 49.2 |
| 3 | 10.0 (14.0%) | 27.5 (38.5%) | 5.0 (7.0%) | 25.0 (35.0%) | 3.8 (5.3%) | 51.0 |
| 4a | 12.5 (18.8%) | 17.5 (26.2%) | 7.5 (11.2%) | 10.0 (15.0%) | 20.0 (30.0%) | 50.0 |
| 4b | 17.5 (21.7%) | 15.0 (18.6%) | 8.0 (9.9%) | 15.0 (18.6%) | 25.0 (31.0%) | 48.0 |
Each synthesized polysaccharide (100 µg) was digested with a mixture of heparin lyases. The resultant disaccharides were purified by a BioGel P-2 column, and resolved by reversed-phase ion-pair partition high-performance liquid chromatograph.
The amount of each disaccharide was estimated by determining its peak area with a standard curve generated with known amount of the disaccharide standard.
A recovery yield was calculated by using 2-O-[35S]sulfo heparin as an internal standard.
ΔUA, 4-deoxy-α-L-threo-hex-4-enopyranosyluronic acid; GlcNAc, N-acetylglucosamine; GlcNS, N-sulfoglucosamine; ΔUA2S, 4-deoxy-α-L-threo-hex-4eno-2-sulfopyranosyluronic acid; GlcNS6S, N-sulfo-6-sulfoglucosamine.