Figure 2. Overexpression of lin-3 induces ectopic gland cell death(s) through let-23 and the core PCD pathway.

(A) Monomeric red fluorescent protein (mRFP) images of the wild-type, syIs1, and ced-3(n717); syIs1 worms expressing P_B0280.7::4Xnls::mrfp are shown. The arrowheads indicate cells expressing mRFP. The percentage of animals losing gland cell(s) is shown in the bottom right corner. **indicates P<0.001 when compared to wild-type (Fisher's exact test). One hundred L4 animals were scored for each genotype. The image stacks were merged by maximum intensity projection using Image J. Scale bar: 10 µm. (B) The percentage of worms losing gland cell(s) of the indicated genotypes was shown. **indicates P<0.001 (Fisher's exact test). More than forty worms were scored for each genotype.