Figure 3. Effect of amino acids preceding PPX on pausing efficiency.

Various sequences were introduced at the area coding for the N-terminus of GFP. When possible, sequences of genes found to either pause or not pause in E. coli were used (names in parenthesis). A) PP-basic, B) PP-acid and PP-hydrophobic, C) PP-amide, D) PP-hydroxy and E) PP-Pro. The mean of at least three biological replicates is shown and error bars indicate one standard deviation.