Table 2. Proteins co-purified with MTR4-GFP and identified by LC-MS/MS.
Experiment 1 | Experiment 2 | Experiment 3 | ||||||
Accession | NAME or Annotation | Function | Mascot score | #Spectra | Mascot score | #Spectra | Mascot score | #Spectra |
AT1G59760 | MTR4 (bait) | 7666.5 | 1332 | 7928.1 | 1352 | 8755.6 | 1999 | |
AT3G21540 | Transducin family/WD-40 repeat protein | Ribo. bio. | 537.8 | 26 | 606.2 | 29 | 720.0 | 28 |
AT1G15440 | Periodic tryptophan protein 2 | Ribo. bio. | 718.5 | 27 | 863.7 | 27 | 857.8 | 26 |
AT3G61620 | RRP41 | EXO9 | 548.2 | 21 | 503.5 | 21 | 592.3 | 26 |
AT1G63810 | Nrap family, nucleolar protein 6 alpha | Ribo. bio. | 563.3 | 18 | 449.4 | 10 | 907.6 | 26 |
AT5G11240 | Transducin family/WD-40 repeat protein | 653.5 | 24 | 647.8 | 24 | 730.1 | 24 | |
AT3G06530 | U3 small nucleolar RNA-associated protein 10 domain | Ribo. bio. | 476.1 | 17 | 597.5 | 19 | 762.8 | 24 |
AT4G04940 | Transducin family/WD-40 repeat protein | Ribo. bio. | 692.4 | 26 | 576.2 | 22 | 713.4 | 23 |
AT5G38890 | CSL4 | EXO9 | 360.8 | 14 | 507.5 | 23 | 545.0 | 22 |
AT3G07750 | RRP42 | EXO9 | 580.3 | 23 | 487.8 | 19 | 576.4 | 22 |
AT4G07410 | POPCORN | Auxin signaling | 464.9 | 12 | 430.6 | 17 | 618.9 | 21 |
AT2G18900 | Transducin family/WD-40 repeat protein | 468.6 | 15 | 371.5 | 14 | 639.3 | 21 | |
AT3G57940 | unknown protein, DUF1726 | Nuc. bind. | 491.5 | 11 | 577.3 | 19 | 737.6 | 19 |
AT5G16750 | TORMOZ EMBRYO DEFECTIVE | Ribo. bio. | 486.4 | 8 | 611.3 | 13 | 728.9 | 19 |
AT5G22100 | RNA cyclase family protein | Ribo. bio. | 443.5 | 12 | 441.1 | 16 | 518.3 | 17 |
AT2G25355 | RRP40 | EXO9 | 429.7 | 15 | 363.8 | 11 | 505.8 | 17 |
AT4G27490 | MTR3 | EXO9 | 355.5 | 13 | 331.3 | 15 | 342.5 | 15 |
AT1G10490 | unknown protein, DUF1726 | Nuc. bind. | 454.7 | 11 | 479.9 | 14 | 589.9 | 15 |
AT1G60080 | RRP43 | EXO9 | 565.1 | 15 | 360.3 | 12 | 558.1 | 15 |
AT1G03360 | RRP4 | EXO9 | 228.7 | 7 | 192.2 | 5 | 361.8 | 15 |
AT4G05410 | YAOZHE | Ribo. bio. | 441.3 | 15 | 422.7 | 13 | 372.9 | 13 |
AT3G60500 | RRP45B/CER7 | EXO9 | 341.9 | 12 | 267.2 | 7 | 474.8 | 12 |
AT1G63780 | IMP4, putative U3 small nucleolar ribonucleoprotein | Ribo. bio. | 268.3 | 9 | 272.9 | 11 | 307.7 | 11 |
AT4G28450 | WD-40 repeat protein | Nuc. bind. | 251.6 | 11 | 312.6 | 10 | 285.1 | 11 |
AT2G47990 | SLOW WALKER1 | Ribo. bio. | 293.0 | 7 | 266.0 | 6 | 358.5 | 11 |
AT1G06720 | BMS1 domain protein | Ribo. bio. | 269.4 | 5 | 319.4 | 4 | 430.2 | 10 |
AT4G30990 | ARM repeat family protein | 296.8 | 7 | 334.0 | 6 | 348.0 | 9 | |
AT3G10530 | Transducin/WD40 repeat protein | Nuc. bind. | 265.2 | 7 | 249.1 | 5 | 298.7 | 9 |
AT4G28200 | U3 small nucleolar RNA-associated protein 6 family | Ribo. bio. | 338.9 | 7 | 293.3 | 6 | 285.9 | 6 |
AT3G46210 | RRP46 | EXO9 | 136.5 | 5 | 147.9 | 8 | 154.2 | 5 |
AT5G30495 | Fcf2 pre-rRNA processing protein | Ribo. bio. | 90.1 | 3 | 113.1 | 6 | 138.8 | 5 |
AT2G36930 | Zinc finger (C2H2 type) family protein | Nuc. bind. | 75.9 | 1 | 70.8 | 4 | 108.2 | 5 |
AT5G15750 | Ribosomal protein S4 family protein | Ribo. bio. | 178.6 | 4 | 255.0 | 8 | 237.8 | 4 |
AT1G69070 | Nop14-like protein | Ribo. bio. | 282.6 | 6 | 112.5 | 3 | 241.9 | 4 |
AT2G46230 | PIN domain-like family protein | Ribo. bio. | 120.1 | 5 | 42.2 | 1 | 105.0 | 4 |
AT1G18850 | unknown protein | 141.1 | 3 | 96.4 | 1 | 181.5 | 4 | |
AT1G30880 | unknown protein | 128.2 | 4 | 139.5 | 3 | 126.4 | 3 | |
AT4G02400 | U3 ribonucleoprotein (Utp) family protein | Ribo. bio. | 157.7 | 4 | 98.1 | 2 | 119.9 | 3 |
AT1G31660 | ESSENTIAL NUCLEAR PROTEIN 1 | Ribo. bio. | 163.9 | 6 | 66.4 | 1 | 139.1 | 3 |
AT1G15420 | contains small-subunit processome Utp12 domain | Ribo. bio. | 80.8 | 2 | 64.0 | 1 | 112.7 | 3 |
AT4G25340 | FK506 BINDING PROTEIN 53 | Ribo. bio. | 112.2 | 3 | 60.9 | 1 | 64.4 | 2 |
AT3G57000 | nucleolar essential protein-related, EMG1/NEP1 domain | Ribo. bio. | 130.7 | 3 | 77.4 | 4 | 39.2 | 1 |
AT2G17250 | NUCLEOLAR COMPLEX ASSOCIATED 4 | Ribo. bio. | 50.6 | 3 | 119.7 | 4 | 43.8 | 1 |
AT2G03820 | NONSENSE-MEDIATED MRNA DECAY 3 | Ribo. bio. | 35.2 | 1 | 67.7 | 1 | 110.3 | 1 |
Ribo. bio., predicted or known function in ribosome biogenesis; EXO9, subunit of exosome core complex, Nuc.bind., Nucleotide binding.