Figure 4. Identification of allelic linkage with invasive growth from the BY4741-by-SK1 cross.
A) Summary of phenotypic screening results. B) A chromosomal plot indicating non-synonymous allelic variation and LOD scores is presented for chromosome XV, highlighting a region of clustered high LOD scores (>4) around the SFL1 and MDM32 loci. This region is boxed in red, and the constituent genes are indicated with the nucleotide positions of the allelic difference; for genes that contain multiple allelic differences, a nucleotide range is listed encompassing the respective residues. C) The MDM32 locus is highlighted in a plot of LOD score by chromosomal position, and the inset box indicates the orientation of MDM32 and neighboring genes on chromosome XV. LOD scores are indicated for each gene in the boxed region, and as in Figure 2, the width of the LOD score bar indicates the chromosomal boundaries of the allelic changes. D) Specific nucleotide and amino acid changes between BY4741- and SK1-encoded alleles of the SFL1 and MDM32 genes are indicated, along with the respective LOD score for each gene.