Figure 8. Reactive oxygen species (ROS) production by neutrophils.

Neutrophils were cultured in complete RPMI at 2×10⁶ cells per well in a 6-well plate for 2 h. Adherent neutrophils were recovered and re-seeded at 2×10⁵ cells per well in a 96-well plate in PBS containing Ca²⁺ and Mg²⁺ in the presence or absence of DPI (10 µM). Neutrophils were pretreated with luminol (200 µM) for 20 min before infecting with bacteria (MOI = 5) pre-incubated with the indicated non-immune serum concentrations. Luminescence was assessed beginning at 40 s post-infection, with detection every 20 s for 10 min. * p<0.05 compared to respective 0% as determined by one-way ANOVA; error bars represent standard error mean. (n = 4).