Figure 11. Effect of antibody and complement opsonization on ROS production by neutrophils.
Neutrophils were cultured in complete RPMI at 2×106 cells per well in a 6-well plate for 2 h. Adherent neutrophils reseeded at 2×105 cells per well in a 96-well plate and assessed for luminescence after treatment with Bp or Bt opsonized with the indicated concentrations of normal serum and/or antisera (1∶200), as described in Figure 8. * p<0.05 compared to Bp 0%, ** p<0.05 compared to Bt 0% as determined by one-way ANOVA; error bars represent standard error mean. (n = 3).