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A) Varying amounts (starting at 4.5×109 VP/mouse) of Ad5 or Ad5-gp83 were immobilized into the wells of ELISA plates and incubated with His6 MAb. B) Either Ad5 or Ad5-gp83 at 6×108 VP of were immobilized on an ELISA plate followed by varying dilutions of monoclonal antibody to His6 (starting at 1∶2,000 dilution). The binding was detected with a HRP-conjugated secondary antibody. OD was read at 450 nm with a microplate reader.
