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. 2014 Aug 21;8(8):e3089. doi: 10.1371/journal.pntd.0003089

Figure 4. Antigen capsid-incorporation vector elicits an in vivo T. cruzi isotype-specific response.

Figure 4

Post-prime, post-boost, and post-reboost of BALB/c and C57BL/6 mice (n = 7) serum was used for the isotype-specific assays. A–B) Ten µM of purified gp83 (KIYWKQPVEGTKSWTLSK) antigenic peptide was bound to ELISA plates. Residual unbound peptide was washed from the plates. The plates were then incubated with immunized mice serum followed by isotype-specific antibodies. The binding was detected with HRP conjugated secondary antibodies. OD at 450 nm represents isotype-specific T. cruzi gp83 antibody levels in sera. The values are expressed as the mean ± standard deviation. (*) = P≤0.05, and (**) = P≤0.01. C–D) Pie charts illustrating the isotype distribution patterns post-prime, post-boost, and post-reboost for immunized BALB/c mice or C57BL/6 sera.