Skip to main content
. 2014 Aug 21;8(8):e3103. doi: 10.1371/journal.pntd.0003103

Figure 1. Characterization of pGui1 and pGui2 in the pathogenic L.interrogans strain Gui44 by PFGE and Southern blot.

Figure 1

PFGE and Southern Blot (using radiolabeled spGui1 probe) of undigested plasmid DNA (lanes A1 and B1); plasmid DNA digested with NotI (A2 and B2); plasmid DNA digested with BamHI (A3 and B3); total genomic DNA digested with NotI (A4 and B4); and undigested total genomic DNA (A5 and B5). The fragment in A2 and B2 indicates the linearized pGui1 (∼78 kb). The corresponding fragment presents in A4 and B4. The larger fragment in A3 indicates the linearized pGui2. The smaller fragment in A4 is one fragment of pGui1 digested with BamHI. Salmonella Braenderup (H9812) digested with XbaI and is used as the molecular weight size standard (M).