Figure 4. Presence of CD4⁺CD45RO⁺ T cells in the DC-CD4⁺CD45RA⁺ T cell co-culture influences Th1 read-out parameters.

(A) CD4⁺, CD4⁺CD45RO⁺, CD4⁺CD45RA⁺ populations have been isolated by negative immunomagnetic separation from freshly isolated PBMC. IFN-γ production of total CD4⁺ (black square), CD4⁺CD45RA⁺ (light gray circle), and CD4⁺CD45RO⁺ (dark gray triangle) T cells co-cultured with FMKp/IFN-γ-matured DC for 7 days as measured by CBA. (B) Contribution of contaminating CD4⁺CD45RO⁺ T cells (2.5, 5 or 10%) to CD4⁺CD45RA⁺-derived IFN-γ-production compared with pure (>99.9%) CD4⁺CD45RA⁺ T cell populations. Data shown are representative of 2 independent experiments.