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. 2014 Aug 21;9(8):e102301. doi: 10.1371/journal.pone.0102301

Figure 6. Subcellular localization and tissue specific expression of IBR5.

Figure 6

a) Sub-cellular localization of IBR5.1 and IBR5.3 isoforms. 35S::IBR5.1-GFP and 35S::IBR5.3-GFP reporter constructs were transiently expressed in Nicotiana benthamiana leaves and images were acquired using Olympus FV1000 confocal microscopy. b) Expression of IBR5.1-GFP in Arabidopsis seedlings. The IBR5::IBR5.1-GFP translational reporter construct was stably expressed in Arabidopsis. Expression of IBR5.1-GFP in root cells was detected using Olympus FV1000 confocal microscopy. (c–j) Tissue specific expression of IBR5.1-GUS in Arabidopsis seedlings. The IBR5::IBR5-GUS translational reporter construct was used to examine tissue specific expression of IBR5. Seven day old light grown seedlings were fixed and stained for GUS. IBR5 expression in the (c) cotyledons and top region of the hypocotyl, (d) elongation zone of the root tip, (e) shoot-root junction, (f) pericycle and endodermis of the mature region of the root, (g–j) adjacent pericycle and endodermis cells of lateral root initiation sites.