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. 2014 Aug 21;9(8):e105306. doi: 10.1371/journal.pone.0105306

Figure 2. Expression of TWIST1, SNAIL, PDCD4, vimentin and E-cadherin were regulated by H. pylori cytotoxin-associated gene A (CagA) in gastric cancer cells.

Figure 2

CagA full-length plasmid was transfected into AGS, SGC-7901 and BGC-823 cells for 48 h,and cells were harvested for mRNA and western blot analysis. (A–C) Quantitative RT-PCR analysis of mRNA level of PDCD4, EMT-related transcription factors (TWIST1 and SNAIL) and cell markers(vimentin and E-cadherin)in 3 gastric cancer cell lines. All values were normalized to β- actin mRNA in the same sample. (D) Western blot analysis of protein level of CagA, TWIST1, SNAIL, PDCD4, vimentin and E-cadherin in control or CagA-transfected cells. (E–G) Western blot densitometry was quantified (ImageJ) and the level of the indicated protein was normalized to β-actin. Data shown represent mean±SD of 3 independent experiments. * P<0.05,** P<0.01 versus control.