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. 2014 Aug 21;9(8):e105306. doi: 10.1371/journal.pone.0105306

Figure 3. PDCD4 regulated EMT-related transcription factors and cell markers in gastric cancer cell lines.

Figure 3

Plasmids of pEGFP-C1 and pEGFP-C1-PDCD4 were respectively transfected into AGS, SGC-7901 and BGC-823 cells for 48 h, and then cells were harvested for isolating total cellular RNA or protein. (A–C) qRT-PCR analysis of mRNA level of TWIST1, SNAIL, PDCD4, vimentin and E-cadherin in AGS, SGC-7901 and BGC-823 cells. All values were normalized to β-actin mRNA in the same sample. (D) Western blot analysis of 5 target proteins expression in control cells and PDCD4 overexpression cells. (E–G) Western blot results were quantified (ImageJ) and the level of the target protein was normalized to β-actin. Data are mean±SD from 3 independent experiments. * P<0.05,** P<0.01 versus pEGFP-C1 con.