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. 2014 Aug 21;9(8):e105306. doi: 10.1371/journal.pone.0105306

Figure 4. H. pylori CagA promoted EMT in part by regulating PDCD4.

Figure 4

All experiments were divided into three groups: 1) pCDNA3.1+pEGFP-C1 (control), 2) pCDNA3.1-CagA+pEGFP-C1, 3) pCDNA3.1-CagA+pEGFP-C1-PDCD4. According to various groups, the corresponding plasmids were transfected into AGS, SGC-7901 and BGC-823 cells. Then cells were harvested for mRNA and western blot analysis. (A–C) qRT-PCR analysis of mRNA level of TWIST1, SNAIL, PDCD4, vimentin and E-cadherin in gastric cancer cell lines. All values were normalized to β- actin mRNA in the same sample. (D) Western blot analysis of CagA, TWIST1, SNAIL, PDCD4, vimentin and E-cadherin protein expression in all cells. (E–G) Western blot results were quantified (ImageJ) and normalized to β-actin. Data shown represent mean±SD from 3 independent experiments. * P<0.05, ** P<0.01 versus con, Δ P<0.05,ΔΔ P<0.01 versus pEGFP + CagA.