Figure 1. Augmentation of IgA production by LG2055 in vitro and in vivo.

Whole PP cells were cultured with or without each of the four Lactobacillus strains (LG2055, L. gasseri JCM1131^t (LG1131T), L. helveticus SBT2171 (LH2171), L. acidophilus SBT2062 (LA2062), 10 µg/ml) for 7 days (A). Whole PP cells were cultured with 0, 0.1, 1.0, and 10 µg/ml of LG2055 for 7 days (B). Whole PP cells were cultured with or without LG2055 (10 µg/ml) for 3, 5, and 7 days (C) The amounts of IgA in culture supernatants were determined by ELISA. Each experiment was done with tripricate cultures; data are shown as the mean ± SD. The values for cells cultured with lactic acid bacteria are compared with that of without the bacteria by one-way ANOVA, Dunnett's post test (A and B) and the t-test (C). Significant differences are indicated by * P<0.05, ** P<0.01, *** P<0.001. LG2055 was orally administrated to BALB/c mice for 5 weeks. Amounts of total IgA in intestinal tissue extracts (D) were determined by ELISA. The population of IgA⁺ B220⁺ cells in PP cells (E) and IgA⁺ B220^- cells in LP cells (F) was analyzed by FACS. Representative data from two independent experiments are shown. Data are shown as the mean ± SD (number of mice n = 10). Significant difference from control group at *P<0.05, **P<0.01 was shown by the t-test (D, E, and F).