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. 2014 Aug 21;9(8):e105589. doi: 10.1371/journal.pone.0105589

Figure 6. Thermal effect of light application on PC simple spike rates in tgGabra6-cre mice lacking ChR2 expression.

Figure 6

PC simple spike rate responds to light application in tissue after expression of the tdTomato specifically in granule cells using AAV2/1.CAG-Flex.tdTomato. (A) Average response profile of 20 PCs showing increased activity during the time course of prolonged light application. Since no ChR2 is present, the observed increase in PC firing must be related to warming of the tissue surrounding the glass fiber. Light was delivered at maximum output power of the laser (20 mW), corresponding to approximately 5 mW measured in front of the tip. Bold black line indicates mean time course of rate modulation averaged across 20 PCs, 20 light applications per cell. (B) Increase in PC simple spike rate during light activation of cerebellar tissue expressing tdTomato in GC. Average activity increased from 47.5 to 54.4 Hz during light application.