Skip to main content
. 2014 Aug 21;10(8):e1004322. doi: 10.1371/journal.ppat.1004322

Figure 1. Knockdown of BNL2a, BILF1 and BGLF5 in transduced LCLs.

Figure 1

A) qRT-PCR was performed to measure the relative knockdown of BNLF2a transcript levels in shControl- and shBNLF2a-LCLs. BNLF2a-mRNA expression was normalized against BZLF1 and shown as relative BNLF2a expression. (B) BNLF2a protein knockdown was assessed using western blot analysis. Protein levels of BNLF2a and BZLF1 was measured in shControl- and shBNLF2a- LCLs. C) qRT-PCR assay of BGLF5 expression normalized against BZLF1 transcript level. Data are shown as BGLF5 expression relative to shControl LCLs. D) BGLF5 knockdown was confirmed at the protein level using western blot analysis. The expression of BGLF5 and BZLF1 protein was measured in shControl-LCLs and shBGLF5-LCLs. E) qRT-PCR assay of BILF1 expression normalized against BZLF1 transcript. Data are shown as BILF1 expression relative to that in shControl-LCLs.