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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: J Immunol. 2014 Jan 3;192(3):1100–1106. doi: 10.4049/jimmunol.1302515

Figure 3. Framework revertants of antibody VRC01.

Figure 3

(A) Sequence alignment (top: heavy chain; bottom: light chain) of a putative germline version, designed partial framework revertants, and mature VRC01. Residues that are identical to germline are shown as dots. CDR mutations are shown in yellow on grey background; framework mutations are colored according to the variant in which they are first introduced (orange – closest to germline; purple; blue – mature). For each residue position, the respective distance to antigen and CDRs is shown. (B) Mapping of framework mutations from germline backbone onto a VRC01 complex structure (coloring same as in A). (C) Selection criteria for framework mutations.