FIGURE 2.

Reduced chromatin accessibility at the major FXN-TSS in FRDA. A, the major FXN-TSS (FXN-TSS2 at −59), which maps within an NDR (DNase I-hypersensitive cluster in ENCODE), is depicted with an arrow in boldface type. The region analyzed for chromatin accessibility, which includes FXN-TSS2, is indicated by a horizontal line. B, relative chromatin accessibility (%) is significantly reduced in FRDA versus non-FRDA (CNTR) cells. Two control regions are shown that do not show any difference in chromatin accessibility in FRDA versus non-FRDA cells; a constitutively accessible region of the GAPDH gene, and a region of the RHO gene that is known to be inaccessible. These data represent the cumulative results from two complete experiments using three FRDA and three non-FRDA (CNTR) lymphoblast cell lines, with each assayed in triplicate. Error bars represent ± S.E. **, p < 0.01.