FIGURE 7.

MBTH assay response to mole fraction of p-ABA in 0.3 mm combined concentration of p-ABA and p-HABA simulating increasing yields of p-ABA produced from p-HABA. Solutions totaling 0.3 mm p-ABA plus p-HABA but varying with respect to the mole fraction of p-ABA were augmented with 1.25 mm NADH and 17.5 μm NR and incubated for 15 min at 29 °C before being assayed for aromatic amine content using MBTH. The mole fraction is expressed as a function of the amine content: [p-ABA]/([p-ABA] + [p-HABA]).