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. 2014 Apr 17;289(22):15215–15230. doi: 10.1074/jbc.M113.544080

FIGURE 6.

FIGURE 6.

Representative NMR spectra of E2p(1–190) didomain in the absence and presence of E1p. Top, two-dimensional 1H-15N HSQC TROSY spectrum of E2p(1–190) didomain (0.5 mm, in red) overlaid with that of the E2p(1–190) didomain (0.5 mm)-E1p (0.12 mm with 0.24 mm ThDP/pyruvate) subcomplex (in blue) recorded at 25 °C in 20 mm KH2PO4 (pH 7.0) with 0.15 m NaCl. Assigned peaks are indicated with one-letter amino acid code and residue number. Bottom, chemical shift deviation in LDh upon complexation of E2p(1–190) didomain with E1p.