TABLE 1.

List of primers used for introducing point mutations in pdr5 gene on pRE5 plasmid

S is sense, and AS is antisense.

Mutation	Direction	Sequence, 5′ to 3′
P195S	S	GCTAGTCGTTTTAGGTAGATCAGGCTCTGGCAAAAC
	AS	GTTTTGCCAGAGCCTGATCTACCTAAAACGACTAGC
C199K	S	CGTTTTAGGTAGACCAGGCTCTGGCAAAACTACTTTATTAAAATCCATCTCTTC
	AS	GAAGAGATGGATTTTAATAAAGTAGTTTTGCCAGAGC CTGGTCTACCTAAAACG
N334E	S	GATCCAAATTTCAATGCTGGGATGAAGCTACAAGGG GTTTGGATTC
	AS	GAATCCAAACCCCTTGTAGCTTCATCCCAGCATTGAA ATTTGGATC
Y367H	S	CTGCCACAGTGGCCATCCATCAATGTTCTCAAGATG
	AS	CATCTTGAGAACATTGATGGATGGCCACTGTGGCAG
N1011S	S	GTTGTTGGTGTTGCTGGTGAAGGTTTATCTGTTGAAC AAAGAAAAAGATTAACCATTG
	AS	CAATGGTTAATCTTTTTCTTTGTTCAACAGATAAACCT TCACCAGCAACACCAACAAC
V1012G	S	GTTGCTGGTGAAGGTTTAAACGGTGAACAAAGAAAA AGATTAACC
	AS	GGTTAATCTTTTTCTTTGTTCACCGTTTAAACCTTCAC CAGCAAC
E1013G	S	GTTGCTGGTGAAGGTTTAAACGTTGGTCAAAGAAAAA GATTAACCATTGGTG
	AS	CACCAATGGTTAATCTTTTTCTTTGACCAACGTTTAAA CCTTCACCAGCAAC