FIGURE 2.

Knockdown of PRMT5 results in reduced levels of CXCL10 at the transcriptional level. A, CXCL10 pre-mRNA levels were measured following TNF-α-stimulation (p < 0.005). B, CXCL10 mRNA stability was quantified following stimulation (3 h) and application of actinomycin D (1 μg/ml). C, CXCL10 promoter activity was measured by luciferase luminescence intensity (p < 0.005). D, ELISA was used to measure levels of CXCL10 in conditioned medium (p < 0.0005; *, p < 0.05; **, p < 0.01; ***, p < 0.005). Error bars represent S.E. (n = 3).