FIGURE 4.

NF-κB p65 is methylated by PRMT5. A, immunoprecipitated endogenous p65 was probed by Western blot for the SDMA modification following TNF-α stimulation. Densitometry of the SDMA signal is shown at right (p > 0.05). B, endogenous p65 was immunoprecipitated from control and siPRMT5-transfected cells and probed for SDMA by Western blot (left) and quantified by densitometry (right; p < 0.01). C, in vitro methyltransferase assay was analyzed by Western blot with anti-SDMA antibody (left) and quantified in the right panel (p < 0.01). D, endogenous p65 was immunoprecipitated from cytosolic and nuclear extracts and probed with the anti-SDMA antibody. SAM, S-adenosylmethionine; IB, immunoblot; **, p < 0.01. Error bars represent S.E. of three independent experiments.