PRMT5 is necessary for p65 association with the CXCL10 promoter.
A–C, in a ChIP assay, protein-DNA complexes were immunoprecipitated with anti-p65 antibody from control and PRMT5 siRNA-transfected, TNF-α-stimulated EC. A, enrichment of the CXCL10 promoter was enhanced upon TNF-α stimulation in control-transfected cells (p < 0.05), but not in PRMT5-depleted conditions. B, p65 association with the CX3CL1 promoter was also increased upon application of TNF-α in control transfected cells (p < 0.05). C, association of p65 with the MCP-1 promoter was not altered by PRMT5 depletion. *, p < 0.05; **, p < 0.01. Error bars represent S.E. of three independent experiments.