SDMA-p65 is critical for CXCL10 induction.
A, p65 arginine-to-lysine mutants at Arg-30, Arg-35, Arg-174, Arg-304, and Arg-330 were expressed and immunoblotted for p65. Quantification of the immunoblot is shown at the right (p > 0.05). B, lysates from p65 3′-UTR targeting siRNA and control transfection are presented (left) and quantified (right; p < 0.05). C–E, knockdown of endogenous p65 and reconstitution with R30K, R35K, R174K, R304K, and R330K was performed in EC. TNF-α-mediated induction of CXCL10 (C), CX3CL1 (D), and MCP-1 (E) in EC reconstituted with p65 mutants is shown. *, p < 0.05; **, p < 0.01; ***, p < 0.005. Error bars represent S.E. of 3–4 independent experiments.