Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Apr 15;289(22):15363–15373. doi: 10.1074/jbc.M114.562785

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 1. — Immunopurification of rat brain Nav1.2 from control and seizure animals. A, immunoblot analysis of Nav1.2 and Kv2.1 in control (C) and seizure (S) RBM samples (10 μg of protein/lane). B, solubilization of Nav1.2 in 1% Triton X-100 detergent extract. T, total RBM starting material; S, detergent-soluble fraction; I, detergent-insoluble fraction. C, immunoblot analysis of Nav1.2 IP. Input, 20 μg of protein; FT, flow-through fraction from immunopurification; IP, IP fraction. Fractions were each 10 μg of protein. D, colloidal Coomassie Blue staining of IP products from control and seizure brain. Nav, Nav channel band excised for analysis. Hspa5, IgG binding protein. mAb, mAb IgG heavy chain. Numbers to the left of panels refer to M_r standards.