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. 2014 Apr 15;289(22):15363–15373. doi: 10.1074/jbc.M114.562785

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Phosphorylation (pS554) and Arg methylation (MeR563) in the Nav1.2 ID I-II linker region are reciprocally regulated in control and seizure samples. A, volcano plot of Nav1.2 peptides from the region 552–570 containing phosphorylation and MeArg. B–D, examples of label-free quantification of peptides that are either unmodified (B), phosphorylated at Ser(P)-554 (C), or methylated at MeArg-563 (D) between control (solid line) and seizure (dashed line) samples. The x axis shows the retention time (min) of peptides on C18-column liquid chromatography, and parentheses with italic type indicate the charge state of each peptide.