FIGURE 3.

Amino acid alignment of avian scFv and germ-line sequence. The chicken scFvs were numbered by the Chothia, Chothia with structurally corrected framework indels, and Kabat schemes. The framework regions (FR) and CDRs are indicated below the numbering schemes, with the associated CDR definitions for each scheme indicated by the red-shaded areas. Variations in amino sequence from the germ-line (for scFv 180 and B8) are highlighted in gray for clarity, insertions are indicated by the yellow boxes, and gaps are illustrated by dashes. The sequences of the V_L and V_H of the avian Fab PDB code 4GLR are shown at the bottom for comparison (19). The chicken mature light chain is two residues shorter than that of the typical mammalian λ light chain and, therefore, begins at position number 3. The antibodies were numbered using “Abysis” (53).