FIGURE 2.
Recognition of GroMM by Mincle mutants. A, mouse-human chimera (closed triangles) containing the cytoplasmic (CYT) and transmembrane (TM) domains of mouse Mincle as well as the extracellular (EXT) domain of human Mincle, and the human-mouse chimera (open triangles) containing the CYT and TM domains of human Mincle as well as the EXT domain of mouse Mincle were expressed in NFAT-GFP reporter cells and tested for their ability to recognize TDM and GroMM. NFAT-GFP reporter cells expressing either human wild-type Mincle (closed circles) or mouse wild-type Mincle (open circles) as well as those expressing FcRγ alone (indicated with x) were also included as controls. B, NFAT-GFP reporter cells expressing either the mousehu174–176 mutant with Val-Leu-Val to Ala-Thr-Leu mutations at positions 174–176 or the mousehu195–196 mutant with Ile-Pro to Val-Thr mutations at positions 195–196 were obtained and analyzed as in A. Assays were performed in triplicate, and the mean values and S.D. are shown.