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. 2014 Apr 13;289(22):15405–15412. doi: 10.1074/jbc.M114.566489

FIGURE 5.

FIGURE 5.

Mincle-dependent recognition of GroMM by human macrophages. A, responses of human Mincle/FcRγ-expressing NFAT-GFP reporter cells to plate-coated TDM (10 ng/well) (left panel) and GroMM (100 ng/well) (right panel) were evaluated in the presence of serially diluted hybridoma cell supernatants of either the 1H2 clone (closed circles) or a negative control clone (open circles). The responses observed without the addition of the supernatants (−) were defined as 100%, and the relative values are shown for each response. B, production of TNF-α by human macrophages in response to plate-coated TDM and GroMM (1 or 5 μg/well) as well as 100 ng/ml LPS was assessed. C, response of human macrophages to plate-coated GroMM (1 μg/well) was assessed in the presence or absence of either 1H2 ascites or negative control ascites (P3). Assays were performed in triplicate, and the mean values and S.D. are shown.