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. 2014 Apr 16;289(22):15463–15473. doi: 10.1074/jbc.M114.561019

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FIGURE 6. — Histone modifications are facilitated by unga overexpression. A and B, representative confocal fluorescence images of embryos following co-immunostaining using anti-H3K4me3 (A) or anti-H3K27me3 (B) together with anti-H3 antibodies. Embryos were injected at the one-cell stage with 500 pg of mcherry (mch) or unga-mcherry (unga-mch) mRNA and fixed at 256-cell and sphere stages for immunostaining. C and D, relative immunostaining intensity of H3K4me3 (C) and H3K27me3 (D) normalized to H3. Ne, number of measured embryos; Nc, total number of measured nuclei. **, p < 0.05; ***, p < 0.01. Error bars, S.D.