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. 2014 Apr 16;289(22):15463–15473. doi: 10.1074/jbc.M114.561019

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — unga knockdown or overexpression affects nuclear transcriptional activity. Embryos at the one-cell stage were injected with 5 pmol of BrUTP in combination with 10 ng of cMO, 10 ng of unga-MO, 500 pg of mcherry (mch) mRNA or 500 pg of unga-mcherry (unga-mch) mRNA and fixed at indicated stages for immunostaining with anti-BrUTP and anti-H3 antibodies. The immunostained embryos were imaged by confocal microscopy. The relative transcriptional activity is the ratio of BrUTP to H3 signal intensity in the nucleus. A and B, representative confocal images (A) and the average relative transcriptional activity (B) in MO-injected embryos. C and D, representative confocal images (C) and the average relative transcriptional activity (D) in mRNA-injected embryos. In image panels, a selected nucleus (indicated by an arrow) in the representative embryo is enlarged underneath. Ne, number of measured embryos; Nc, total number of measured nuclei. ***, p < 0.01. Error bars, S.D.